Introduction to microscopy - online resources
This is a list of good microscopy 101 resources for new Holden lab arrivals, which may be useful to others too. Thanks to the sci-twitter community for pointing to or donating many of these resources.
Basic first overview of principles of fluorescence microscopy from Nikon. They have lots of other good microscopy training resources on the same site. Holden lab newbies, look at this first.
Outstanding, comprehensive online courses on Microscopy :
For quick overviews:
- General microscopy introduction: BioImagingtoolbox- Guillaume Jacquemet
- Introduction to microscope optics: Basics of Optical Microscopy - Nicolas Olivier
- Introduction to image analysis: Image_analysis_lecture__Vincent_Boudreau
- Introduction to advanced microscopy for microbiology - Seamus Holden (with a few slides borrowed from Nicolas Olivier’s lecture above).
- Introduction to microscopy (with a focus on neuroscience): https://www.scribd.com/document/360138012/Master-Course-2017-Photonic-Imaging-in-Neurobiology-C-Leterrier
- Introduction to reproducible and ethical acquisition and analysis of microscopy data are outlined in these two papers: - Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images - A guide to accurate reporting in digital image acquisition - can anyone replicate your microscopy data?
More detailed microscopy lecture series:
- Jeff Lichtmann light microscopy lectures (excellent intro to microscopy with a focus on optics):
- Oxford Micron facility Advanced light microscopy course: http://downloads.micron.ox.ac.uk/lectures/micron_course_2017/
Microscope manufacturer tutorials:
Other commercial resources:
Key textbooks
- “Principles of Fluorescence Spectroscopy” by Lakowicz, the reference text for fluorescence microscopy. (a private copy for lab members is on our server)
- Single-Molecule Techniques: A Laboratory Manual, Editor TJ Ha, an excellent single molecule protocols book, a little dated but still very useful