VerCINI

Bacterial traps for high resolution microscopy

Over several years we have developed a method called Vertical Cell Imaging by Nanostructured Immobilization (VerCINI), together with the Cees Dekker lab. It turns out that by immobilizing bacteria upright in nanofabricated cell traps, we can take much higher resolution images of any process organized along the cells short axis, especially bacterial cell wall synthesis.

To support other researchers in adopting this technology, we wrote a detailed protocols paper. We also teamed up with a nanofabrication company called ConScience, who can manufacture the silicon wafers required to make VerCINI cell traps, for modest price and without any profit to us. If you are interested in obtaining one of these wafers, please contact Joachim Fritze at ConScience.

References

2023

  1. Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis
    Stuart Middlemiss, David M Roberts, James Grimshaw, and 6 more authors
    bioRxiv, 2023
  2. A one-track model for spatiotemporal coordination of Bacillus subtilis septal cell wall synthesis
    Kevin D Whitley, James Grimshaw, Eleni Karinou, and 2 more authors
    bioRxiv, 2023

2022

  1. High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI)
    Kevin D Whitley, Stuart Middlemiss, Calum Jukes, and 2 more authors
    Nature Protocols, 2022

2021

  1. FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division
    Kevin D Whitley, Calum Jukes, Nicholas Tregidgo, and 6 more authors
    Nature communications, 2021

2017

  1. Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
    Alexandre W Bisson-Filho, Yen-Pang Hsu, Georgia R Squyres, and 8 more authors
    Science, 2017